ᐊ P4-48
Open Readings 2021 • P4-49
P5-2 ᐅ

(IN-SITU) STUDY OF IMMOBILIZED RECEPTOR BINDING KINETICS BY USING PLANAR PHOTONIC-PLASMONIC NANOSTRUCTURES FOR BIOSENSING

Vytautas Gradauskas1, Justina Anulytė1, Ernesta Bužavaitė-Vertelienė1, Ieva Plikusienė1, 2, Zigmas Balevičius1, 3

1 State Research Institute Center for Physical Sciences and Technology, Sauletekio ave. 3, LT-10257 Vilnius, Lithuania

2 Faculty of Chemistry and Geoscience, Vilnius University, Naugarduko str. 24, LT-03225 Vilnius, Lithuania

3 Faculty of Electronics, Vilnius Gediminas Technical University, Naugarduko str. 41, LT-03227 Vilnius, Lithuania

[email protected]

Biosensors based on surface plasmon resonance (SPR) are widely used in biosensing due to the high sensitivity of the SPR. However, various photonic-plasmonic structures are investigated in order to reduce the high losses in the metal, that broadens the width of plasmonic resonance, and as a result, decreasing sensitivity of the sensor. In order to reduce the losses in the metal a modified photonic-plasmonic nanostructure, supporting Tamm plasmon-surface plasmon polaritons (TPP-SPP) [1] mode could be employed for detection of protein interactions on solid-liquid interfaces [2, 3]. Such type of plasmonic mode consists of two different plasmonic excitations: the so called Tamm plasmon-polariton (TPP) and surface plasmon-polariton (SPP). The SPP mode is generated at metal/dielectric interface as the TPP is generated at the PC/metal boundary in the photonic band gap of PC.

In this research a photonic-plasmonic nanostructure consisting of PC (TiO2/SiO2) with thin (~40 nm) Au layer was investigated. A total internal reflection ellipsometry (TIRE) method for generation of plasmonic modes were employed. A stem cell factor receptor (SCF-R) and bovine serum albumin (BSA) were used in order to investigate the sensitivity of the plasmonic modes. The kinetics interactions of immobilized SCF-R receptor on the chip surface with genetically engineered ligands was studied. The results showed a higher concentration detection limit on a TPP-SPP mode, rather that of SPR.

[1] E. Bužavaitė-Vertelienė, V. Vertelis and Z. Balevičius, The experimental evidence of a strong coupling regime in the hybrid Tamm plasmon-surface plasmon polariton mode, Nanophotonics, 2021. https://doi.org/10.1515/nanoph-2020-0660

[2] E. Buzavaite-Verteliene, I. Plikusiene, T. Tolenis et. al., Hybrid Tamm-surface plasmon polariton mode for highly sensitive detection of protein interactions, Opt. Express 28, 29033-29043 (2020).

[3] Z. Balevičius, Strong coupling between Tamm and surface plasmons for advanced optical biosensing, Coatings, 10(12), 1187 (2020).