Flax (Linum usitatissimum L.) is a valuable medicinal plant rich in bioactive compounds, including lignans and flavonoids, which contribute to its antioxidant properties. In vitro callus cultures provide a controlled system for the production of secondary metabolites, yet their antioxidant potential remains largely unexplored. This study aims to evaluate the phytochemical composition and antioxidant activity of flax callus cultures [1]. Callus was induced from seeds on Murashige and Skoog (MS) medium supplemented with auxin 1-naphthylactic acid and cytokinin 6-benzylaminopurine. By adding the amino acids L-phenylalanine and L-tryptophan. For comparison, flax grown in a nutrient medium without growth regulators was studied (Figure 1). %20Callus%20Cultures%20In%20Vitro.jpg)
The antioxidant activity of extracts was assessed using DPPH, FRAP, ABTS assays, while total phenolic, flavonoid and protein content was quantified spectrophotometrically. Additionally, antioxidative enzyme activity were analyzed to further evaluate the biochemical properties of the extracts. Preliminary results indicate that antioxidative activity, flavonoid, and phenolic content showed significant enhancement with the addition of L-tryptophan, while protein content reached its highest levels when supplemented with L-phenylalanine. These findings emphasize the potential of flax callus cultures as a sustainable source of bioactive compounds, particularly for pharmaceutical and cosmetic applications. Future research will focus on optimizing culture conditions to further enhance the accumulation of these valuable metabolites.