Open Readings 2025 • P4-90
BIOSYNTHESIS OF SILVER NANOPARTICLES VIA MEDUSOMYCES GISEVII FERMENTATION WITH TILIA CORDATA EXTRACT: ANTIMICROBIAL, ANTIOXIDANT PROPERTIES AND PHYTOCHEMICAL ANALYSIS.
Urte Raubyte1, Aiste Balciunaitiene1, Mindaugas Liaudanskas2, Jonas Viskelis1
1 Institute of Horticulture, Lithuanian Research Centre for Agriculture and Forestry, 54333 Babtai, Lithuania
2 Department of Pharmacognosy, Faculty of Pharmacy, Lithuanian University of Health Sciences, 50166 Kaunas, Lithuania
[email protected]
Nanotechnology is one of the most advanced fields in the twentieth century, with diverse research areas and applications [1, 2]. Multiple nanoobjects with simultaneous action capability often possess both antioxidant and antimicrobial qualities, in this case emphasizing silver nanoparticles with a size range of 1-100 nm, which exhibit high antimicrobial properties as they interact strongly with low concentrations of microorganisms. Silver ions initiate several mechanisms that disrupt natural cell processes and metabolites, such as the cell’s protein structure, the activity of the respiratory chain, cell membrane structure, and deoxyribonucleic acid (DNA) replication. Synthesis of silver nanoparticles (AgNPs) can be achieved through various methods – microwave and biological, photochemical, chemical, physical, and microemulsion. When it comes to biological synthesis, reducing agents often are microorganisms and parts of plants. The therapeutic properties of the flowers and leaves of Tilia species (Linden) contribute to their significance in pharmacogenetic research. Tilia cordata, rich in phenolic compounds, flavonoids, and tiliroside, has been traditionally used for its sedative, diuretic, and antibacterial properties, with its extracts showing potential in treating anxiety, hypertension, respiratory infections, and oxidative stress-related conditions. The goal of our study was to investigate the significance of green nanotechnology in the development of a cost-effective and eco-friendly approach for the biosynthesis of silver nanoparticles utilizing Tilia cordata extract. This process aimed to enhance the antioxidant and antimicrobial activities of the synthesized nanoparticles.
To quantify the total phenolic content (mg GAE/g) in the ethanol extracts of Tilia cordata, the Folin–Ciocâlteu method was used. The highest concentration of phenolic compounds was observed in the composition of fermented T. cordata AgNPs extract. In contrast, a significantly lower phenolic content (18%) was detected in the pure T. cordata herb extract. Furthermore, the data indicate that following the synthesis of silver nanoparticles, the phenolic compound concentration decreased to 206.00±0.59 mg GAE/g ( 2%). This reduction in phenolic content is likely attributed to the role of phenolic compounds as reducing agents and stabilizers. The pure aqueous extract of T. cordata herb demonstrated antioxidant properties across all applied analytical methods, including Cupric Reducing Antioxidant Capacity (CUPRAC), Ferric Reducing Antioxidant Power (FRAP) assay, and Ultra-High Performance Liquid Chromatography (UHPLC) coupled with mass spectrometry. The surface morphology and chemical composition of AgNPs were analysed using Scanning Electron Microscopy (SEM). SEM micrographs of fermented T. cordata showed uniformly distributed nanoparticles without irregularities or holes. Transmission Electron Microscopy (TEM) images revealed diverse morphologies, predominantly spherical o The authors would like to thank the Research Council of Lithuania, (LMTLT) agreement No. S-MIP-24-56, for the financial support given to achieve this work.
[1] Mashau, M. E.; Mamagau, T.; Foforane, K.; Nethathe, B.; Ramphinwa, M. L.; Mudau, F. N. Biosynthesis of Silver Nanoparticles from Fermented Bush Tea (Athrixia Phylicoides DC) Leaf Extract and Evaluation of Their Antioxidant and Antimicrobial Properties. Fermentation 2023, 9 (7), 648. https://doi.org/10.3390/fermentation9070648.
[2] Balciunaitiene, A.; Januskevice, V.; Saunoriute, S.; Raubyte, U.; Viskelis, J.; Memvanga, P. B.; Viskelis, P. Antimicrobial Antioxidant Polymer Films with Green Silver Nanoparticles from Symphyti Radix. Polymers 2024, 16 (3), 317. https://doi.org/10.3390/polym16030317.
[3] Balciunaitiene, A.; Puzeryte, V.; Radenkovs, V.; Krasnova, I.; Memvanga, P. B.; Viskelis, P.; Streimikyte, P.; Viskelis, J. Sustainable–Green Synthesis of Silver Nanoparticles Using Aqueous Hyssopus Officinalis and Calendula Officinalis Extracts and Their Antioxidant and Antibacterial Activities. Molecules 2022, 27 (22), 7700. https://doi.org/10.3390/molecules27227700.
[4] Ramphinwa, M. L.; Mchau, G. R. A.; Madala, N. E.; Nengovhela, N.; Ogola, J. B. O.; Mudau, F. N. Response of Plant Growth and Development, and Accumulation of Hydroxyl-Cinnamoyl Acid Derivatives to Selected Shade Nets and Seasonality of Field-Grown Bush Tea (Athrixia Phylicoides DC.). HortScience 2022, 57 (1), 87–96. https://doi.org/10.21273/HORTSCI16171-21.
[5] Hashemi, Z.; Mizwari, Z. M.; Mohammadi-Aghdam, S.; Mortazavi-Derazkola, S.; Ali Ebrahimzadeh, M. Sustainable Green Synthesis of Silver Nanoparticles Using Sambucus Ebulus Phenolic Extract (AgNPs@SEE): Optimization and Assessment of Photocatalytic Degradation of Methyl Orange and Their in Vitro Antibacterial and Anticancer Activity. Arab. J. Chem. 2022, 15 (1), 103525. https://doi.org/10.1016/j.arabjc.2021.103525.
[6] Chen, J.; Wang, J.; Zhang, X.; Jin, Y. Microwave-Assisted Green Synthesis of Silver Nanoparticles by Carboxymethyl Cellulose Sodium and Silver Nitrate. Mater. Chem. Phys. 2008, 108 (2–3), 421–424. https://doi.org/10.1016/j.matchemphys.2007.10.019.
[7] Sharma, V. K.; Yngard, R. A.; Lin, Y. Silver Nanoparticles: Green Synthesis and Their Antimicrobial Activities. Adv. Colloid Interface Sci. 2009, 145 (1–2), 83–96. https://doi.org/10.1016/j.cis.2008.09.002.
[8] Zehra, S. H.; Khan, S. A.; Zehra, S. M.; Asad, M. Z. Removal of Cr6+ from Synthetic Polluted Water Using Fe Modified Sugarcane Bagasse & Peanut Shell Powder. J. Chem. Environ. 2023, 2 (2), 1–13. https://doi.org/10.56946/jce.v2i2.146
[9] Ghosh Chaudhuri, R.; Paria, S. Core/Shell Nanoparticles: Classes, Properties, Synthesis Mechanisms, Characterization, and Applications. Chem. Rev. 2012, 112 (4), 2373–2433. https://doi.org/10.1021/cr100449n.
[10] Amith Yadav, H. J.; Eraiah, B.; Kalasad, M.N.; M. Thippeswamy, M.; Rajasreelatha, V. Synthesis, Characterization of ZrO2:Tb3+ (1-9 Mol %) Nanophosphors for Blue Lighting Applications and Antibacterial Property. Biointerface Res. Appl. Chem. 2021, 12 (6), 7147–7158. https://doi.org/10.33263/BRIAC126.71477158.
