Open Readings 2025 • P4-58
ELECTROCHEMICAL FORMATION AND EVALUATION OF POLYPYRROLE LAYER FOR DEVELOPMENT OF MOLECULARLY IMPRINTED POLYMER
Domas Pirstelis1, 2, Ernestas Brazys2, Vilma Ratautaite1, Arunas Ramanavicius1, 2
1 Department of Nanotechnology, State Research Institute Center for Physical Sciences and Technology, Sauletekio Ave. 3, Vilnius LT-10257, Lithuania
2 Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko str. 24, Vilnius LT-03225, Lithuania
[email protected]
Electrically conductive polymers, such as polypyrrole (Ppy), have been widely utilized in the field of molecular imprinting‘ for use in biosensors [1, 2] and drug delivery systems [3]. Herein, we demonstrate electrochemical synthesis and in situ monitoring of a polypyrrole film for its application as a molecularly imprinted polymer (MIP )-based electrochemical sensor.
In this study, the Ppy film was deposited on a glass slide coated with a layer of fluorine-doped tin oxide (FTO) from an aqueous acetate buffer solution. The polymer was electrochemically synthesized using the pulsed chronoamperometry (PCA) method. The formation and changes to the Ppy layer were monitored in situ utilizing fast Fourier transform electrochemical impedance spectroscopy (FFT-EIS). Changes in cumulative charge Q and constant phase element admittance Y\(_{CPE}\) were observed during the formation of the layer.
The results demonstrate the viability of synthesizing Ppy films in an acetate buffer. The possibility of closely monitoring and controlling their characteristics is also demonstrated. This project has received funding from the Research Council of Lithuania (LMTLT), agreement No. [S-MIP-24-111].
[1] V. Liustrovaite, V. Ratautaite, A. Ramanaviciene, and A. Ramanavicius, “Detection of the SARS-CoV-2 nucleoprotein by electrochemical biosensor based on molecularly imprinted polypyrrole formed on self-assembled monolayer,” Biosens Bioelectron, vol. 272, p. 117092, Mar. 2025, doi: 10.1016/J.BIOS.2024.117092.
[2] V. Ratautaite, E. Brazys, A. Ramanaviciene, and A. Ramanavicius, “Electrochemical sensors based on L-tryptophan molecularly imprinted polypyrrole and polyaniline,” Journal of Electroanalytical Chemistry, vol. 917, Jul. 2022, doi: 10.1016/j.jelechem.2022.116389.
[3] J. Sarvutiene, U. Prentice, S. Ramanavicius, and A. Ramanavicius, “Molecular imprinting technology for biomedical applications,” Mar. 01, 2024, Elsevier Inc. doi: 10.1016/j.biotechadv.2024.108318.
