RESEARCH OF GENOTOXIC EFFECTS INDUCED BY POLYSTYRENE NANOPARTICLES IN ALLIUM CEPA ROOT MERISTEM CELLS

Agnė Kilnaitė1, Asta Stapulionytė1

1 Institute of Biosciences, Life Sciences Center, Vilnius University, Lithuania

[email protected]

Polystyrene nanoparticles, also known as nanoplastics, have become a significant environmental issue in recent years due to their increasing abundance and potential harm to the environment and wildlife. They can come from a variety of sources, including the breakdown of larger plastic products and the release of microbeads from personal care products. The small size of nanoplastics allows them to evade filtration systems and persist in the environment, including water and soil. This signifies that they can remain in the environment for a long time, can be transported over large distances by wind, water, and other means and can easily enter the food chain [1]. Overall, the problem of polystyrene nanoparticles is serious because of the potential harm they can cause to wildlife, human health, and the environment.

Plants play a crucial role in the ecosystem, and understanding the effects of nanoparticles on their cells can provide valuable insights into the impact of these particles on the environment and the food chain. To further understand the potential genotoxic effects of polystyrene nanoparticles, a cytogenetic study on Allium cepa root meristem cells was conducted. The common onion is generally used as a model organism in cytogenetic studies due to its well-studied root system and ease of cultivation [2]. Solutions containing various concentrations of polystyrene nanoparticles were tested and the length of onion roots was examined. A plant bioassay, called Allium test, was applied to determine the mutagenic potential of polystyrene nanoparticles, ratio of chromosome aberrations, mitotic index and micronuclei frequencies were observed and compared with results of the negative control.

The findings of this study are expected to contribute to a better understanding of the potential dangers of polystyrene nanoparticles and their impact on the ecosystem. By investigating the effects of these particles on the environment, the study aims to provide valuable insights into the need for proper disposal methods and to raise awareness about the importance of environmental conservation.


[1] Gigault, J., Halle, A. T., Baudrimont, M., Pascal, P.-Y., Gauffre, F., Phi, T.-L., El Hadri, H., Grassl, B., & Reynaud, S. (2018). Current opinion: What is a nanoplastic? Environmental Pollution (Barking, Essex: 1987), 235, 1030-1034.

[2] Leme, D. M., & Marin-Morales, M. A. (2009). Allium cepa test in environmental monitoring: A review on its application. Mutation Research/Reviews in Mutation Research, 682(1), 71-81.