Fibroblasts are the most common connective tissue cells, which can be found abundantly e.g. in reticular, loose or dense connective tissue forming structures like lymph nodes, tendons or skin layer. By producing reticular, collagen and elastic fibers but also components of the basic substance, they play an important role in wound healing and scar formation process [1]. This special properties of fibroblasts can be useful in the treatment of hard to heal wounds and tissue fractures. Research on the use of fibroblasts in the treatment of wounds has been conducted for many years [2,3,4]. Production of extracellular matrix components via fibroblasts can be also apply as alternative treatment in restoring the natural elasticity of scarred skin.
In order to understand and use the knowledge of how to treat wounds and scars with fibroblasts in veterinary medicine, a method of collecting, isolating and cultivating fibroblasts from tissue should be developed.
Isolation of cell line was conducted by collecting the material, and then via mechanical fragmentation of the material, cleaning the collected skin sample. The next step is transferring the material to test tubes with the medium that is stored in the incubator in a humidified atmosphere of 95% air and 5% CO2 for 3 h at 37 °C. DMEM medium was used for cell culture, every 48h medium was changed.
The skin tissue was taken during the procedure mastectomy, from a Maltese dog, at age 6.