MICROFLUIDIC CHIP FABRICATION FOR ELECTROPORATION OF CELLS

Microfluidics is a new technique to produce high sensitivity, high speed, high throughput, and cost-efficient analysis by manipulating fluids using microfabricated channel and chamber structures. Additionally, its use in high-level chemical, biological and physical analyses and affordable point-of-care assays are rapidly expanding [1]. The capacity to regulate cell microenvironment factors at pertinent length and time scales is one of microfluidics' main advantages [2]. Microfluidics and the creation of microfluidic chips is key to development of molecular sensors based on bioassays, such as immunoassay, cell separation, DNA amplification, imitation of biological and physiological conditions, membrane systems and other analysis. Microfluidic chip channels and chambers usually are made via high precision methodologies such as lithography and then transferred to a castable material such as silicone (polydimethylsulixane - PDMS) or other. Currently, due to rapid expansion in desktop resin printers, fast prototyping of microfluidic devices or small scale production became much more accessible. Herein, we present PDMS casting methodologies and tooling for advanced, high quality, and dimensional accuracy PDMS microfluidic devices for electroporation of cells and fast laboratory oriented applications. Methodologies are oriented for secondary clear resin casting (such polymers as Off-stoichiometry thiol-ene polymer) using PDMS master molds for microfluidic device manufacturing.