Environmental chemical stressors cause oxidative stress in aquatic organism. The consequences of oxidative damage include modifications of lipids and carbohydrates, protein denaturation, DNA and RNA damage. The aim of the study was to investigate the biomarkers of oxidative stress in the larvae of rainbow trout (Oncorhynchus mykiss) after exposure to tire fire effluents. The biochemical status of antioxidant defences of rainbow trout (O. mykiss) larvae, which is a valuable model fish for monitoring water pollution, were studied after 4 days exposure to tire fire effluents. In addition, 8 days recovery experiment was carried out. As biochemical biomarkers of toxicity and oxidative stress, metallothioneins (MT) induction and catalase (CAT) activity were analysed. Metallothioneins level was measured by colorimetric reaction using Elman reagent. Catalase activity was assessed by a modified colorimetric reaction. Protein content was determined by the Bradford method. Fish larvae were exposed to tire fire effluents at 100% and 12.5% concentrations. The results showed that tire fire effluents resulted in decreased amount of MT and increased activity of CAT in O. mykiss larvae. The data obtained showed that after 8 days of recovery MT amount increased to a control level. Accordingly, CAT activity reached a level of control in all recovery groups. This study highlights the ecotoxicological potential of tire fire accidents to the aquatic biota.
TOXICOLOGICAL EFFECTS OF TIRE FIRE EFFLUENTS: CATALASE AND METALLOTHIONEIN INDUCTION IN RAINBOW TROUT (Oncorhynchus mykiss) LARVAE
Lina Anulevičiūtė1, 2, Gintarė Sauliutė2, Arvydas Markuckas1, Živilė Jurgelėnė2, Milda Stankevičiūtė2
1 Vilnius University, Life Sciences Center, Saulėtekio av. 7, 10223 Vilnius, Lithuania
2 Nature Research Centre, Akademijos St. 2, LT-08412 Vilnius, Lithuania
Acknowledgments: This study was funded by the Research Council of Lithuania through the project P-MIP-21-229.